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VBScript (short for Visual Basic Scripting Edition) is an easy-to-use scripting language developed by Microsoft that enables authors to create powerful tools using a subset of the Visual Basic language. VBScript is implemented as a fast, portable interpreter for web browsers and applications that use ActiveX controls and OLE Automation servers.
rhinoceros 3d portable
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Now, the question is, will iRhino 3D remain a handy portable viewing and mark-up tool, or play a wider, more creative role in conceptual design? That, of course, will be down to users. I wonder how far away we are from Grasshopper running on an iPad?
The northern white rhinoceros (NWR, Ceratotherium simum cottoni) is the most endangered mammal in the world with only two females surviving. Here we adapt existing assisted reproduction techniques (ART) to fertilize Southern White Rhinoceros (SWR) oocytes with NWR spermatozoa. We show that rhinoceros oocytes can be repeatedly recovered from live SWR females by transrectal ovum pick-up, matured, fertilized by intracytoplasmic sperm injection and developed to the blastocyst stage in vitro. Next, we generate hybrid rhinoceros embryos in vitro using gametes of NWR and SWR. We also establish embryonic stem cell lines from the SWR blastocysts. Blastocysts are cryopreserved for later embryo transfer. Our results indicate that ART could be a viable strategy to rescue genes from the iconic, almost extinct, northern white rhinoceros and may also have broader impact if applied with similar success to other endangered large mammalian species.
Here we report the first ever documented generation of blastocyst, pre-implantation rhinoceros embryos through the application of assisted reproduction procedures that had never been previously attempted in this species. Our results suggest that these methods could play a valuable role in the effort to save rhinoceros populations on the brink of extinction. We also obtained embryos from SWR oocytes fertilized with semen of NWR males, clearly indicating the potential of rescuing genes of the NWR sub-species through intra-specific hybrid breeding. Moreover, we report the generation of ES cells with all the features of undifferentiated cells and a high capacity for differentiation in the three lineages. The next challenges will be to transfer the cryopreserved embryos into synchronized fSWR surrogate mothers to establish and carry a pregnancy to term. In parallel, we plan to harvest oocytes from the last two remaining fNWR in Kenya. Currently efforts are underway to achieve the differentiation of rES cells to obtain artificially generated oocytes from banked somatic cells of several NWR individuals using iPS technology4, as a means of achieving sustainable wide genetic diversity.
The authors acknowledge the ZOO Dvůr Králové, Avantea and the Nadace ČEZ for logistic and financial support. We thank our colleagues Arn Ludwig, Dietmar Lieckfeldt, Alessandra Quaggio (IZW), Irina Lagutina, Paola Turini, Andrea Perota (Avantea), Ejona Rusha (Helmholtz Zentrum München), Anje Sporbert, Narasimha Swamy Telugu, Norman Krüger (Max Delbrueck Center), Elke Schuemann, Gundula Thiel, Arno Schnorrenberg, Ivo Weissmann, and Peter Steiner for technical assistance. Steven Seet (IZW) for his activities in regards to fund raising, networking and public relation work for this project. We are thankful to numerous European zoological institutions (listed in alphabetic institutional order) for participating in the advanced assisted breeding program in rhinoceroses: Zoo Budapest, Zoo Chorzów, Zoo Dvůr Kralové, Longleat Safari Park, Montpellier Zoo, Zoo Pairi Daiza, Zoo Poznán, Zoo Salzburg, Zoo Schwerin, Zoo Thoiry. K.H. is supported by KAKEN 15K21736, 25114006, and 17H01395. P.L. is supported by H2020 EU Drynet and EraofArt. We acknowledge the slaughterhouses (L Scordamaglia, CEO of Inalca Spa, Franco Caffi, Vice-President of Prosus Spa and Maria Grazia Ragazzi of Zerbini & Ragazzi SNC) for access to key biological materials. We thank Ms. Brunella Gadda of DHL Express and her team with the logistic of shipping the rhino oocytes. We also thank our colleagues from San Diego Zoo Global for constructive discussions. 2ff7e9595c
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